Anti-Aging Skin Care

The real secret to the anti-aging effects of Lifeline Stem Cell Skincare

By Kim Crawford, M.D. Last updated: June 16, 2020

Lifeline Stem Cell Skincare rocks!

Lifeline stem cell skincare is absolutely the only skincare line that utilizes non-mesenchymal (adult) and non-embryonic stem cell extracts. It also utilizes these cells in a unique way which I’ll explain here. Moreover, I want to give you the science behind these amazing products and will do my best to make it comprehensible.

best anti agin productsThe goal at the Lifeline Stem Cell Skincare headquarters is simple.

They want to improve the look and feel of aging skin. Lifeline® and Lifeline® ProPlus+ Skin Care researchers continue to make new discoveries in the fields of nanotechnology, stem cell biology, and skin products formulation technology.

They create super high-quality, scientifically proven, incredibly effective and, quite frankly, the best anti aging skincare products in the world. Not to toot my own horn here but I co-owned a skin care products company and gave my input regarding ingredients, packaging, and more. I’ve been a real “skin nut” for decades. As a result, I truly know what’s good and what’s great when it comes to skincare products. Now, why don’t we discuss the concept of stem cells and what they can do for aging skin.

Human Stem Cells: Non-Embryonic, Embryonic, Adult

Stem cells are the cells that “get to work” when the skin needs to repair itself. The Lifeline stem cell skincare products actually contain the peptides, growth factors, cytokines, and enzymes which are extracted from stem cells.

Lifeline®’s parent company, International Stem Cell Corporation (ISCC) owns the patent on the way to take human eggs (oocytes) and stimulate them to begin dividing, without fertilization occurring. These stem cells are called parthenogenetic stem cells (“partheno” comes from the Greek word for virgin, and “genetic” means birth). Markedly, in this process, an embryo is never created, so it’s also never destroyed. Therefore, this is a uniquely effective yet completely ethical approach (no matter your religious or political views) to creating stem cells.

About Oocytes

The source of these oocytes come from donors who donated their eggs at In Vitro Fertilization clinics. The donors are carefully screened. They must be in their mid-20’s and have good mental and physical health. Their donated eggs are harvested and then basically “tricked” to begin dividing. At this point, we consider these stem cells to be pluripotent. Pluripotent means that they are now capable of being differentiated into different kinds of human cells. Non-embryonic stem cells are the most potent human stem cells that we can use ethically. Take a quick look at the diagram below and then let me explain why indeed that is true. Meanwhile, here’s another quick word about the oocytes.

What is exceptional is the Lifeline scientists have used the same oocyte for 10 years to make their extracts. It was the “winning oocyte”- the best ever donated and here’s why: It conscientiously divides every 30 hours, and then they can retrieve millions of new pluripotent stem cells. This is very important for the consistent potency of the proteins and peptides which are extracted. It’s infinite! In contrast, adult stem cells have a life expectancy, and each time they divide, they make a weaker copy. Using adult stem cells is kind of like what happens with a copy machine. If you have an ink cartridge that makes 1,000 copies, all of the last copies are not as vivid as the first.

lifeline stem cell skincare

The Difference Between the Types of Stem Cells Used in Topical Preparations

Parthenogenetic stem cells

While the stem cells we just discussed are still pluripotent, scientists get to work to extract the anti-aging growth factors, peptides, cytokines and enzymes to use in the Lifeline stem cell skincare serums. Now here is where I’d like you to remember one important fact which I’ll get back to. What I’ll get back to is the REAL secret of why the Lifeline stem cell skincare products are so much more effective than their competitors.

It has to do with the medium the cells are grown in and what is done (or not done) with the medium and the cells by the real experts at Lifeline versus the so-called experts at other companies. It’s a bit “scientific” so I’m saving it for you die-hard science-of-skincare fans. Additionally, if you stick with me, you’ll get a gift!

Petri dish studies have found that the stem cell extracts in Lifeline stem cell skincare products increase the production of important anti-aging proteins including well-known proteins such as collagen and elastin, as well as the lesser-known fibroblast and epidermal growth factors.

Adult Stem Cells

Because the cells are multiple generations from the source, adult stem cells (such as from blood, skin, fat or bone marrow) are less potent and therefore less effective than stem cells closer to the original egg. The growth factors extracted from any adult stem cells have markedly less efficacy regarding any proliferation of new skin cells.

Adult stem cells are in most human organs and tissues, including the skin. If we look at the 5 top selling brands we see (in no particular order as LL is at the top in most reviews including getting their Lifeline Proplus day serum named #1 anti-aging skin care product from Truth in Aging):

TNS by SkinMedica (Allergan)- Human neonatal fibroblasts (not stem cells but they do make cytokines in culture) $270/per ounce

U Autologous by PCS-Your own fat (human adipose derived stem cells) $3000 per ounce (Holy way-too-expensive Batman!)

Blue Horizon-Human placenta & cord blood stem cells-$295/ per ounce

AnteAge by Cellese Regenerative Therapeutics-Adult (youth) human bone marrow-derived mesenchymal stem cells-$150-$200 per ounce

Lifeline by International Stem Cell Corporation-Human parthenogenetic stem cells from an unfertilized embryo-$190 per ounce. Note: This isn’t even the Proplus line which is being re-formulated to be even stronger and will likely be cost-comparable.

Yes, I reviewed, tried and sampled out all of the above before deciding on the Lifeline Stem Cell Skin care line for myself, my patients and my AWS clients. The verdict-much more firmness, fewer lines, and more overall skin appeal! What is the secret of what goes on with the medium?? None of the Lifeline stem cell skincare competitors listed above “do it” right. Not one-stay tuned.

Know Where the Stem Cells Come From Before You Buy Skin Care Products

Lifeline stem cell skincare is a totally new way to treat wrinkles, age spots, and sagging “unfirm” skin. It truly blows away the competition. Here’s another not-the-real-secret-yet reason. Lifeline® Skin Care uses nanotechnology.

By doing so, they can make teensy little sub-microscopic molecules which are able to penetrate down to the epidermal junction. I can’t use the word “dermis” for a non-drug topical product. The products deliver the “good stuff” from the stem cell extracts right to the area where collagen and elastin are made. It is the only stem cell product which delivers the goodies right to where it will be most effective!

anti aging skin care regimen produced these resultstmin Once in the “special place,” the stem cell extracts “communicate” with your skin cells.

This results in a proliferation of new skin cells.

Scientific studies confirm what you will see in the mirror.

A 77% reduction in wrinkle length and depth in 3 months. You will also see lifting, firmness, and brightness (luminescence). Your skin will just plain look and behave like younger skin. In fact, 90% of study participants noticed firmer, tighter skin. In addition, 59% saw a visible reduction of lines and wrinkles. 87% said that their appearance was noticeably improved. If these products are not part of your anti aging skin care regimen, why not?

How to tighten skin without surgery? It’s the only way we know. What about the types of acne scars that are not too deep but still bothersome, will these products help? Yes, indeed. So, it’s now time for the real, scientific secret that sets this product light years apart, as if what you just read didn’t do that already!

The secret

It’s about the process of how the cells grow, what they grow in, and what they do with the two things I mention above. The process is a bit complex, but I’ll break it down step by step.

How stem cells are grown and nourished

It is “accepted science” that stem cells are grown in petri dishes which contain a complex “goo” of nourishing things we’ll call culture media. In return, that culture media is immediately filled with cellular products which are ejected by the stem cell cytoplasm.

The stem cells eject what they wish to try to control their environment into the culture media. This culture media is then dubbed “conditioned media.” The cellular products ejected into the “conditioned media” are comprised of a small number of “helpful” things like growth factors, as well as cellular waste products which can have deleterious effects on your skin.

Virtually every single stem cell product on the market OTHER THAN Lifeline Stem Cell skincare products are made using stem cells grown and harvested in conditioned media and what’s worse, the companies which do this, advertise it to the unknowing public as if it’s a GOOD THING! “Conditioned media is the name given to the “spent” nutrient broth in which cells have been cultured. It contains the biosignals produced by the cells during culture” according to the Anteage folks who then use this conditioned media to make their products– same as the “other guys.”

I’ll get back to why it’s most definitely not a good method but first will tell you how Lifeline does it right.

How to do this right:
Step One

As soon a the parthenogenic stem cells undergo a few divisions, the Lifeline scientists scoop them out of the culture media before they have a chance to “spit out” anything. This is then a cellular extract which then turns into a cellular lysate preparation. So, what does this mean? In short, lysing cells means breaking open the cells and separating the soluble components (proteins) from the non-soluble ones (cell membranes, fragments of organoids, chromosomes).

They are thereby extracting all of the factors present in the cytoplasm, (not just what the cells choose to excrete). This produces a much more concentrated solution than the conditioned media.


The next step is to “keep the good” and “eliminate the bad.” This step is essential because the lysate liquid may contain over 5000 different proteins and peptides including some undesirable substances such as DNA, unsoluble molecules, large molecular weight biopolymers, etc.

The unwanted substances are removed using an ultracentrifuge, leaving only the low molecular weight proteins and peptides (which are just smaller protein molecules). This is what Lifeline then calls their usable cellular extract, consisting of a mixture of approximately 2,000 proteins and peptides. About 100 of these are found in significant concentrations. The most important of these proteins are the growth factors (FGF2, EGF< KGF, TGFB3) and the collagen and elastin stimulating/promoting proteins. They know these proteins and peptides are present in significant amounts from the effects seen in gene expression, and that they have a beneficial effect when applied to skin cells.

Step Three

The last step in the process is to stabilize the extract. This means that they then take even more time and care to remove water from around the proteins so that they don’t have a degradation in efficacy over time. As a result, this gives the finished products a stable “shelf life” and eliminates the chance of bacteria.

Finally, as a part of the last step, they encapsulate the liposomes which does several beneficial things. It stunts and prevents bacterial growth, protects the important protein factors and antioxidants from UV or oxidative damage and serves as a more effective delivery system to further improve absorption of active ingredients.

LL takes these extra steps, and they are still the most cost-effective of the leading products! Amazing!

Why don’t other companies do this?

serious skin carWell, let’s see-could it be, uh-money??? What do you think? I vote yes. It’s quite tempting for companies to sell products containing conditioned media because it is much cheaper to produce and the cells can produce large quantities of it every 48 hours or so.

The cells are not sacrificed to harvest the medium, and one batch of cells can produce many batches of the conditioned medium before it needs to be thrown away.

However, it is clearly not possible to be as potent or effective and may be potentially harmful due to the waste products. Or, even if the waste products merely cancel out the effects of the growth factors, why would you want to use a product which announces that it contains cellular waste products?

Yuk. That’s how I feel about it.

By the way, the waste products are very difficult to remove from conditioned media, so perhaps these other companies have tried and failed? I don’t know the answer. It’s either that or they didn’t bother because you the consumer would never know the difference if I weren’t writing this article.


To summarize, if you are puzzling over where to spend your money regarding stem cell-growth factor products I hope this article helps. I hope you see these Lifeline stem cell skin care products are what we call amazing anti aging skincare. If you have an interest in anti-aging skin care, then you’ll love my article on how to look young forever without having plastic surgery. Enjoy!


  1. What a great explanation. I’m now going to put this to the test!

  2. Thank you for treating we readers like intelligent life forms. This is the definitive blog on growth factors and cultured medium and so on. I’m now going to purchase the LLPP duo and have high hopes!

FREE email consultation with Dr. Kim included with every purchase & FREE shipping on all orders over $150